Precipitating antibodies to Aspergillus spp

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Altogether 55 patients suffering from IPA were investigated. The diagnosis was made on the basis of combined clinical, immunological, histological, mycological studies of patients subjected to a hospital treatment in mycology department of Betanien clinic and outpatiently.
Diagnoses were made using criteria of invasive mycoses diagnosing, developed by the European institution of cancer study and treatment and Mycology research group of the Institute of Allergy and Infectious Diseases of USA.
Clinical methods of investigations included examination by a therapist, clinical and biochemical blood tests, fiber-optic bronchoscopy, study of external respiration function. Microbiological study was carried out in accordance with standard practice. To reveal supersensitiveness of an organism to possible allergens, the researchers applied a method of skin testing and revealing of specific IgE in blood serum. Allergological testing was carried out in allergology room of mycology department.
To fulfill mycological examination of a phlegm and liquid from bronchi, phlegm was gathered into a sterile container after cleaning of mouth cavity (tooth brushing, gargling with soda liquor), liquid from bronchi was gathered during bronchoscopic investigation.
To carry out histological research the authors used paraffin and frozen section, colored with haematoxylin and eosin, azure II-eosin, Gram-Weigert method, PAS-response, Grocott-Gomori method.
Precipitating antibodies to Aspergillus spp. antigens were defined applying enzyme multiplied immunoassay (EMI). A part of patients was subjected to precipitation reaction.

Total IgE and specific IgE to A.fumigatus was found using methods of radioimmunosorbent test in a nuclear medicine laboratory and enzyme multiplied immunoassay in a laboratory of allergens and allergy diagnostics. Measuring of total IgE was done in kU/l (thousands of international units in a litre, 1 unit = 2.4 ng).